KAT2A Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-18-583
Article Name: KAT2A Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-18-583
Supplier Catalog Number: 18-583
Alternative Catalog Number: PRS-18-583-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human KAT2A (NP_066564.2).
Conjugation: Unconjugated
Alternative Names: Histone acetyltransferase KAT2A, General control of amino acid synthesis protein 5-like 2, Histone acetyltransferase GCN5, HsGCN5, Lysine acetyltransferase 2A, STAF97, KAT2A, GCN5, GCN5L2, HGCN5
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 110kDa
NCBI: 2648
UniProt: Q92830
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:200 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using KAT2A antibody (18-583) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat liver using KAT2A antibody (18-583) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using KAT2A antibody (18-583) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using KAT2A antibody (18-583) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using KAT2A Polyclonal Antibody (18-583) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using KAT2A Polyclonal Antibody (18-583) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using KAT2A Polyclonal Antibody (18-583) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.