HDAC2 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-18-488
Article Name: HDAC2 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-18-488
Supplier Catalog Number: 18-488
Alternative Catalog Number: PRS-18-488-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, IP, WB
Species Reactivity: Human, Monkey, Mouse, Rat
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 390 to the C-terminus of human HDAC2 (NP_001518.3).
Conjugation: Unconjugated
Alternative Names: HDAC2 Antibody: HD2, RPD3, YAF1, Histone deacetylase 2, HD2
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 60kDa
NCBI: 3066
UniProt: Q92769
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using HDAC2 antibody (18-488) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat kidney using HDAC2 antibody (18-488) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using HDAC2 antibody (18-488) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using HDAC2 antibody (18-488) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using HDAC2 antibody (18-488). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of K562 cells using 1ug HDAC2 antibody (18-488). Western blot was performed from the immunoprecipitate using HDAC2 antibody (18-488) at a dilition of 1:1000.