BMPR1B Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-18-442
Article Name: BMPR1B Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-18-442
Supplier Catalog Number: 18-442
Alternative Catalog Number: PRS-18-442-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-126 of human BMPR1B (NP_001194.1).
Conjugation: Unconjugated
Alternative Names: Bone morphogenetic protein receptor type-1B, BMP type-1B receptor, BMPR-1B, CDw293, BMPR1B
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 55kDa
NCBI: 658
UniProt: O00238
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using BMPR1B Antibody (18-442) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Immunohistochemistry of paraffin-embedded human tonsil using BMPR1B antibody (18-442) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using BMPR1B antibody (18-442) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat ovary using BMPR1B antibody (18-442) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using BMPR1B antibody (18-442) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of A431 cells using BMPR1B antibody (18-442) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.