EEF1A1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-18-137
Article Name: EEF1A1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-18-137
Supplier Catalog Number: 18-137
Alternative Catalog Number: PRS-18-137-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 123-462 of human EEF1A1 (NP_001393.1).
Conjugation: Unconjugated
Alternative Names: EEF1A1, CCS-3, CCS3, EEF-1, EEF1A, EF-Tu, EF1A, FLJ25721, GRAF-1EF, HNGC:16303, LENG7, MGC102687, MGC131894, MGC16224, PTI1, eEF1A-1, EE1A1
Clonality: Polyclonal
Concentration: batch dependent
NCBI: 1915
UniProt: P68104
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using EEF1A1 antibody (18-137) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human colon carcinoma using EEF1A1 antibody (18-137) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using EEF1A1 antibody (18-137) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using EEF1A1 antibody (18-137) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using EEF1A1 antibody (18-137) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using EEF1A1 antibody (18-137) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using EEF1A1 antibody (18-137) at dilution of 1:100. Blue: DAPI for nuclear staining.