VDAC1 / Porin Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-16-208
Article Name: VDAC1 / Porin Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-16-208
Supplier Catalog Number: 16-208
Alternative Catalog Number: PRS-16-208-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-283 of human VDAC1 / Porin (NP_003365.1).
Conjugation: Unconjugated
Alternative Names: VDAC1, PORIN, VDAC-1
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 36kDa
NCBI: 7416
UniProt: P21796
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using VDAC1 / Porin antibody (16-208) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat heart using VDAC1 / Porin antibody (16-208) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using VDAC1 / Porin antibody (16-208) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using VDAC1 / Porin antibody (16-208) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH/3T3 cells using VDAC1 / Porin antibody (16-208) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC12 cells using VDAC1 / Porin antibody (16-208) at dilution of 1:100. Blue: DAPI for nuclear staining.