Iba1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-14-395
Article Name: Iba1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-14-395
Supplier Catalog Number: 14-395
Alternative Catalog Number: PRS-14-395-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human Iba1 (NP_001614.3).
Conjugation: Unconjugated
Alternative Names: AIF1, allograft inflammatory factor 1, IBA1, AIF-1, IRT-1, interferon gamma responsive transcript, ionized calcium-binding adapter molecule, Daintain, G1
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 16kDa
NCBI: 199
UniProt: P55008
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
Western blot analysis of extracts of rat spleen, using Iba1 antibody (14-395) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat spinal cord using Iba1 antibody (14-395) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using Iba1 antibody (14-395) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using Iba1 antibody (14-395) at dilution of 1:100 (40x lens).&x0D,
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