SIRT1 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal
Catalog Number:
PRS-13-968
- Images (6)
| Article Name: | SIRT1 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | PRS-13-968 |
| Supplier Catalog Number: | 13-968 |
| Alternative Catalog Number: | PRS-13-968-100 |
| Manufacturer: | ProSci |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC, IP, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 448-747 of human SIRT1 (NP_036370.2). |
| Conjugation: | Unconjugated |
| Alternative Names: | SIRT1 Antibody: SIR2L1, SIR2L1, NAD-dependent protein deacetylase sirtuin-1, Regulatory protein SIR2 homolog 1, hSIRT1 |
| Application Dilute: | Optimal dilutions for each application to be determined by the researcher. |
| Application Notes: | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100 |
|
|
Western blot analysis of extracts of various cell lines, using SIRT1 antibody (13-968) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. |
|
|
Western blot analysis of extracts from normal (control) and SIRT1 knockout (KO) HeLa cells, using SIRT1 antibody (13-968) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. |
|
|
Immunohistochemistry of paraffin-embedded human mammary cancer using SIRT1 antibody (13-968) at dilution of 1:100 (40x lens). |
|
|
Immunohistochemistry of paraffin-embedded human tonsil using SIRT1 antibody (13-968) at dilution of 1:100 (40x lens). |
|
|
Immunofluorescence analysis of HeLa cells using SIRT1 antibody (13-968) at dilution of 1:100. Blue: DAPI for nuclear staining. |
|
|
Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug SIRT1 antibody (13-968). Western blot was performed from the immunoprecipitate using SIRT1 antibody (13-968) at a dilition of 1:1000. |
Product Guarantee and Expert Support
