MYH11 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-13-822
Article Name: MYH11 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-13-822
Supplier Catalog Number: 13-822
Alternative Catalog Number: PRS-13-822-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1680-1979 of human MYH11 (NP_001035203.1).
Conjugation: Unconjugated
Alternative Names: AAT4, FAA4, SMHC, SMMHC, myosin-11, epididymis secretory sperm binding protein, myosin heavy chain, smooth muscle isoform, myosin, heavy chain 11, smooth muscle, myosin, heavy polypeptide 11, smooth muscle
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 250kDa
NCBI: 4629
UniProt: P35749
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using MYH11 antibody (13-822) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human appendix using MYH11 antibody (13-822) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using MYH11 antibody (13-822) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using MYH11 antibody (13-822) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using MYH11 antibody (13-822) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of human placenta cells using MYH11 antibody (13-822) at dilution of 1:100. Blue: DAPI for nuclear staining.