TREM2 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-13-679
Article Name: TREM2 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-13-679
Supplier Catalog Number: 13-679
Alternative Catalog Number: PRS-13-679-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 19-160 of human TREM2 (NP_061838.1).
Conjugation: Unconjugated
Alternative Names: TREM2, triggering receptor expressed on myeloid cells 2, TREM-2, Trem2a, Trem2b, Trem2c, OTTHUMP00000039771, triggering receptor expressed on myeloid cells 2a
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 25kDa
NCBI: 54209
UniProt: Q9NZC2
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:1000IHC: 1:50 - 1:200IF: 1:20 - 1:100
Western blot analysis of extracts of Mouse brain, using TREM2 antibody (13-679) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human tonsil using TREM2 antibody (13-679) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using TREM2 antibody (13-679) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using TREM2 antibody (13-679) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using TREM2 antibody (13-679) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U-251MG cells using TREM2 antibody (13-679) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse lung cells using TREM2 antibody (13-679) at dilution of 1:100. Blue: DAPI for nuclear staining.