MMP16 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-13-634
Article Name: MMP16 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-13-634
Supplier Catalog Number: 13-634
Alternative Catalog Number: PRS-13-634-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 290-565 of human MMP16 (NP_005932.2).
Conjugation: Unconjugated
Alternative Names: MMP16, C8orf57, MMP-X2, MT-MMP3, MT3MMP, MT-MMP 3, MT-MMP2, MT2-MMP, Matrix metalloproteinase-16, Membrane type 3-MMP, MMP-16, MMPX2, MT3-MMP, MTMMP3
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 60kDa
NCBI: 4325
UniProt: P51512
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using MMP16 antibody (13-634) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human lung cancer using MMP16 antibody (13-634) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using MMP16 antibody (13-634) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using MMP16 antibody (13-634) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using MMP16 antibody (13-634) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH/3T3 cells using MMP16 antibody (13-634) at dilution of 1:100. Blue: DAPI for nuclear staining.