Caspase 1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-13-340
Article Name: Caspase 1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-13-340
Supplier Catalog Number: 13-340
Alternative Catalog Number: PRS-13-340-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, IP, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-311 of human Caspase 1 (NP_150635.1).
Conjugation: Unconjugated
Alternative Names: Caspase-1 Antibody: ICE, P45, IL1BC, IL1BCE, Caspase-1, Interleukin-1 beta convertase, CASP-1
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 30kDa/48kDa
NCBI: 834
UniProt: P29466
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using Caspase 1 antibody (13-340) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver cancer using Caspase 1 antibody (13-340) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using Caspase 1 antibody (13-340) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using Caspase 1 antibody (13-340) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of Raw264.7 cells using Caspase 1 Polyclonal Antibody (13-340) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of THP-1 cells, using 3 ug Caspase 1 antibody (13-340). Western blot was performed from the immunoprecipitate using Caspase 1 antibody (13-340) at a dilition of 1:1000.