KU70 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-13-312
Article Name: KU70 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-13-312
Supplier Catalog Number: 13-312
Alternative Catalog Number: PRS-13-312-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Monkey, Mouse, Rat
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 500-600 of human KU70 (NP_001460.1).
Conjugation: Unconjugated
Alternative Names: G22P1, ML8, KU70, TLAA, CTC75, CTCBF, G22P1
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 70kDa
NCBI: 2547
UniProt: P12956
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using KU70 antibody (13-312) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat brain using KU70 Antibody (13-312) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using KU70 Antibody (13-312) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using KU70 Antibody (13-312) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using KU70 Antibody (13-312) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using KU70 antibody (13-312) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.