FGF2 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-13-095
Article Name: FGF2 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-13-095
Supplier Catalog Number: 13-095
Alternative Catalog Number: PRS-13-095-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 143-288 of human FGF2 (NP_001997.5).
Conjugation: Unconjugated
Alternative Names: FGF2, Basic fibroblast growth factor, FGFB, Fibroblast growth factor 2, FGF-2, Prostatropin, BFGF, HBGF-2
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 20kDa
NCBI: 2247
UniProt: P09038
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of rat ovary, using FGF2 antibody (13-095) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat lung using FGF2 antibody (13-095) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using FGF2 antibody (13-095) at dilution of 1:100 (20x lens).
Immunohistochemistry of paraffin-embedded mouse heart using FGF2 antibody (13-095) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using FGF2 Polyclonal Antibody (13-095) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using FGF2 Polyclonal Antibody (13-095) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.