Protein Arks SUMO Protease (Ulp1) specifically targets the tertiary structure of the SUMO moiety, resulting in highly specific cleavage. Following the expression of your SUMO-tagged recombinant protein and cleavage of your protein with Protein Arks SUMO Protease, a native N-terminus will remain on the target protein The removal of the moiety from the solution is straightforward. Affinity chromatography using Ni-NTA resin binds to the poly-His tagged SUMO Protease, allowing your target protein to elute in a pure form. Protein Ark supplies Ni-NTA resin in various configurations to assist in this. Operation ratio is 1:50 - 1:100. Therefore 40µg of SUMO Protease is sufficient for 1-2mg of target protein.
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