The detergent-insoluble fraction of psoriatic human epidermis was used as the immunogen for the Cytokeratin 5/6/18 antibody.
It recognizes polypeptides of 58kDa, 56kDa, and 45kDa, identified as cytokeratin 5, 6, and 18 respectively. It shows no reaction with keratin 1, 8, or 19. This MAb shows a broad pattern of reactivity with epithelial tissues, from simple glandular epithelia to stratified squamous epithelia. Epithelial cells are labeled whether they are ectodermal, mesodermal, or endodermal in origin. This MAb is useful in detecting cells of epithelial origin in mixed tumors, in effusions, in bone marrow samples, or in tissue culture cells.
Clonality:
Monoclonal
Clone Designation:
[LP34]
Purity:
Protein G affinity chromatography
Form:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Antibody Type:
Primary Antibody
Application Dilute:
Immunohistochemistry (FFPE): 0.1-0.2ug/ml
Application Notes:
Optimal dilution of the Cytokeratin 5/6/18 antibody should be determined by the researcher.
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