A recombinant human partial protein corresponding to amino acids 167-261 was used as the immunogen for the PHB antibody.
This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Ant-iproliferative activity is reported to be localized to the 3 UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. [RefSeq]
Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Notes:
The stated application concentrations are suggested starting points. Titration of the PHB antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
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