A portion of amino acids 31-274 were used as the immunogen for the TROP2 antibody.
This intronless gene encodes a carcinoma-associated antigen defined by the monoclonal antibody GA733. This antigen is a member of a family including at least two type I membrane proteins. It transduces an intracellular calcium signal and acts as a cell surface receptor. [Wiki]
Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Notes:
The stated application concentrations are suggested starting points. Titration of the TROP2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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