A C-terminal region peptide was used as the immunogen for the recombinant Cytokeratin 16 antibody.
Cytokeratin 16 is an epidermis-specific type I keratin that plays a key role in skin. Acts as a regulator of innate immunity in response to skin barrier breach: required for some inflammatory checkpoint for the skin barrier maintenance. [UniProt]
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Antibody Type:
Primary Antibody
Application Dilute:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format : incubate for 30 min at RT (1)
Application Notes:
The stated application concentrations are suggested starting points. Titration of the recombinant Cytokeratin 16 antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
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