Napsin A Antibody Cocktail, Clone: [NAPSA/1238 + NAPSA/1239], Mouse, Monoclonal
Biozol Catalog Number:
NSJ-V2997-100UG
Supplier Catalog Number:
V2997-100UG
Alternative Catalog Number:
NSJ-V2997-100UG
Manufacturer:
NSJ Bioreagents
Host:
Mouse
Category:
Antikörper
Application:
FACS, IF, IHC-P, WB
Species Reactivity:
Human
Immunogen:
Amino acids 189-299 from the human protein were used as the immunogen for the mAbs in the Napsin A antibody cocktail.
Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B. There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Antibody Type:
Primary Antibody
Application Dilute:
Flow cytometry: 0.5-1ug/10 6 cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml for 60 min at RT,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Notes:
Optimal dilution of the Napsin A antibody cocktail should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
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