GRP94 Antibody / HSP90B1, Clone: [HSP90B1/1192], Rat, Monoclonal
Biozol Catalog Number:
NSJ-V2910-20UG
Supplier Catalog Number:
V2910-20UG
Alternative Catalog Number:
NSJ-V2910-20UG
Manufacturer:
NSJ Bioreagents
Host:
Rat
Category:
Antikörper
Application:
IHC-P
Species Reactivity:
Human
Immunogen:
Recombinant full-length human protein was used as the immunogen for the GRP94 antibody.
Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This mAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grps are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Antibody Type:
Primary Antibody
Application Dilute:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Notes:
Optimal dilution of the GRP94 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
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