GRP94 Antibody / HSP90B1, Clone: [9G10.F8.2], Rat, Monoclonal
Biozol Catalog Number:
NSJ-V2908SAF-100UG
Supplier Catalog Number:
V2908SAF-100UG
Alternative Catalog Number:
NSJ-V2908SAF-100UG
Manufacturer:
NSJ Bioreagents
Host:
Rat
Category:
Antikörper
Application:
FACS, IF, IHC-P, WB
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Purified glucose regulated protein 94 from chicken oviducts was used as the immunogen for the GRP94 antibody.
Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This mAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grps are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-3ug/ml,Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Notes:
Optimal dilution of the GRP94 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
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