Amino acids 82-98 (DMDARRNKQQRIKEEGE) were used as the immunogen for this p63 antibody.
Tumor Protein p63, also known as KET, is a protein that in humans is encoded by the TP63 gene. Yang et al.(1998) described the cloning of tumor protein, which shows strong homology to the tumor suppressor p53 and the p53-related protein p73. By fluorescence in situ hybridization, Yang et al.(1998) localized the human gene to chromosome 3q27-q29. Hibi et al.(2000) stated that p53 homologs known variously as p40, p51, p63, and p73L (Trink etal., 1998, Yang etal., 1998, Osada etal., 1998, Senoo etal., 1998) are isoforms of the same gene, which were then referred to as AIS for amplified in squamous cell carcinoma.
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Purity:
Antigen affinity
Form:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Antibody Type:
Primary Antibody
Application Dilute:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells
Application Notes:
The stated application concentrations are suggested starting points. Titration of the p63 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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