An amino acid sequence from the C-terminus of human ADIPOQ (LYADNDNDSTFTGFLLYHDTN) was used as the immunogen for this Adiponectin antibody.
Adiponectin, als called ADIPOQ (Adipocyte-, C1q-, and Collagen Domain-Containing), APM1, ADPN and ACDC, is a protein which in humans is encoded by the ADIPOQ gene. Using FISH, Das et al.(2001) mapped the mouse Acrp30 gene to chromosome 16 in a region showing homology of synteny with human 3q27. By RNase protection and Western blot analysis, Schaffler et al.(1999) showed that ADIPOQ is expressed by differentiated adipocytes as a 30-kD protein that is also detectable in serum. By sequence comparisons, they found links between ADIPOQ and TNF family ligands as well as to cytokines expressed by T cells. It is a protein hormone that modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation. It is exclusively secreted from adipose tissue(and also from the placenta in pregnancy) into the bloodstream and is very abundant in plasma relative to many hormones.
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide and 0.025% thimerosal
Purity:
Antigen affinity
Form:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Antibody Type:
Primary Antibody
Application Dilute:
Western blot: 0.5-1ug/ml,IHC (FFPE): 0.5-1ug/ml,IHC (Frozen): 0.5-1ug/ml,ELISA: 0.1-0.5ug/ml (human protein tested)
Application Notes:
The stated application concentrations are suggested starting amounts. Titration of the Adiponectin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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