An amino acid sequence from the C-terminus of human Toll-Interacting Protein (NAQPRCSEEDLKAIQDMFPN) was used as the immunogen for this Tollip antibody.
Toll-Interacting Protein is an inhibitory adaptor protein that in humans is encoded by the TOLLIP gene. Lo et al.(2009) stated that the gene maps to chromosome 11. The mouse gene maps to chromosome 7. By Western blot analysis of embryonic kidney cells, Burns et al.(2000) confirmed the binding of Tollip to IL1RAP, to a complex of IL1RAP-IL1R1, and to IL18R. Burns et al.(2000) proposed that IL1B stimulation induces aggregation of IL1Rs, recruitment of MYD88 followed by Tollip-IRAK complexes, and the phosphorylation of IRAK by MYD88. This leads to the dissociation of Tollip from IRAK, which can then transmit the IL1-induced signals.
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Antibody Type:
Primary Antibody
Application Dilute:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells
Application Notes:
The stated application concentrations are suggested starting amounts. Titration of the Tollip antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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