53BP1 Antibody / Tumor Protein p53-Binding Protein 1, Rabbit, Polyclonal
Biozol Catalog Number:
NSJ-R31082
Supplier Catalog Number:
R31082
Alternative Catalog Number:
NSJ-R31082
Manufacturer:
NSJ Bioreagents
Host:
Rabbit
Category:
Antikörper
Application:
ICC, IHC-P, WB
Species Reactivity:
Human, Mouse, Rat
Immunogen:
An amino acid sequence from the C-terminus of human 53BP1 (ERIGFKQHPKYKHDYVSH) was used as the immunogen for this 53BP1 antibody (100% mouse homology).
Tumor Protein p53-Binding Protein 1, also called 53BP1, is a protein that in humans is encoded by the TP53BP1 gene. Iwabuchi et al. (1998) mapped the gene to 15q15-q21 by FISH. Iwabuchi et al. (1994) showed that TP53BP1 binds to the conformationally sensitive central domain of wildtype p53 but not to mutant p53 in vitro. Immunoblot analysis by Iwabuchi et al. (1998) showed that expression of TP53BP1 or TP53BP2 enhances the transactivation function of p53 and induces the expression of p21 (CDKN1A). Wang et al. (2002) used small interfering RNA directed against the protein in mammalian cells to demonstrate that TP53BP1 is a key transducer of the DNA damage checkpoint signal. It was required for p53 accumulation, G2/M checkpoint arrest, and the intra-S-phase checkpoint in response to ionizing radiation.
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Purity:
Antigen affinity
Form:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Antibody Type:
Primary Antibody
Application Dilute:
Western blot: 0.5-1ug/ml,IHC (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Notes:
The stated application concentrations are suggested starting points. Titration of the 53BP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
* VAT and and shipping costs not included. Errors and price changes excepted
