An amino acid sequence from the middle region of human REA/PHB2 (RPNAQELPSMYQRLGLDY) was used as the immunogen for this Prohibitin 2 antibody (100% homologous in human, mouse and rat).
Prohibitin 2, also called Repressor of Estrogen Receptor Activity (REA), is a protein that in humans is encoded by the PHB2 gene. The International Radiation Hybrid Mapping Consortium mapped the gene to chromosome 12. Montano et al. (1999) showed that REA enhanced the potency of a dominant-negative ER-alpha mutant and antiestrogens as suppressors of ER-alpha activity in Chinese hamster ovary cells. When coexpressed with wildtype ER-alpha or ER-beta (ESR2), PHB2 suppressed activation of a reporter gene in a dose-dependent manner. It had no effect on reporter activity in the absence of liganded ER, and it had no effect on the transcriptional activities of other hormone receptors. Mutation analysis showed that an N-terminal domain and a central domain of PHB2 were required for its repressor activity.
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Purity:
Antigen affinity
Form:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Antibody Type:
Primary Antibody
Application Dilute:
Western blot: 0.5-1ug/ml,IHC (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Notes:
The stated application concentrations are suggested starting amounts. Titration of the Prohibitin 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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