An amino acid sequence from the middle region of human Oxysterol-Binding Protein (WQKSLQYERDQRIRL) was used as the immunogen for this OSBP antibody (100% homologous in human, mouse and rat).
Oxysterol-Binding Protein is a protein that in humans is encoded by the OSBP gene. Im et al.(2005) reported the structure of the full-length yeast oxysterol-binding-protein Osh4, a member of the OSBP-related protein (ORP) family, at 1.5- to 1.9-angstrom resolution in complexes with ergosterol, cholesterol, and 7-, 20- and 25-hydroxy cholesterol. Moreira et al.(2001) refined the localization of the OSBP1 gene to chromosome 11q12.1 by radiation hybrid analysis. Wang et al.(2005) found that OSBP functions as a cholesterol-binding scaffolding protein coordinating the activity of 2 phosphatases to control the extracellular signal-regulated kinase (ERK) signaling pathway. Cytosolic OSBP formed an approximately 440-kD oligomer with a member of the PTPPBS family of tyrosine phosphatases, the serine/threonine phosphatase PP2A, and cholesterol.
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Purity:
Antigen affinity
Form:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Antibody Type:
Primary Antibody
Application Dilute:
Western blot: 0.5-1ug/ml
Application Notes:
The stated application concentrations are suggested starting amounts. Titration of the OSBP antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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