An amino acid sequence from the N-terminus of human Interleukin-1 receptor-associated kinase 4 (DDRYNQFHIRRFEAL) was used as the immunogen for this IRAK4 antibody (100% homologous in human, mouse and rat).
Interleukin-1 receptor-associated kinase 4, also called REN64, is an IRAK family protein kinase involved in signaling innate immune responses from Toll-like receptors. It also supports signaling from T-cell receptors. Scott(2002) mapped the gene to chromosome 12 based on similarity between the REN64 sequence(GenBank AF155118) and a chromosome 12 clone(GenBank AC093012). Functional analysis by Li et al.(2002) determined that IRAK4, like IRAK1 and Pelle, has auto- and cross-phosphorylation kinase activity. Precipitation and binding analyses showed weak interaction between IRAK4 and IRAK1, but IRAK4 did not interact with other IRAK family members. Overexpressed protein interacted with MYD88 and TRAF6 and activated mitogen-activated protein kinase (MAPK) and nuclear factor kappa-B (NFKB) pathways.
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Purity:
Antigen affinity
Form:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Antibody Type:
Primary Antibody
Application Dilute:
Western blot: 0.5-1ug/ml
Application Notes:
The stated application concentrations are suggested starting amounts. Titration of the IRAK4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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