A portion of amino acids 8-39 from the human protein was used as the immunogen for the SIGLEC5 antibody.
SIGLEC5 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. This protein binds equally to alpha2,3-linked and alpha2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. This molecule is expressed by monocytic/myeloid lineage cells. SIGLEC5 is found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. It is expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differenciation.
Western blot: 1:500-1:2000, Immunohistochemistry (FFPE): 1:25
Application Notes:
The stated application concentrations are suggested starting points. Titration of the SIGLEC5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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