A portion of amino acids 269-296 from the mouse protein was used as the immunogen for the MEK1 antibody.
Map2k1 / Mek1 catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Notes:
The stated application concentrations are suggested starting points. Titration of the MEK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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