A portion of amino acids 180-207 from the human protein were used as the immunogen for the VIL1 antibody.
Villin-1 is a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments.
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25,Flow cytometry: 1:25 (1x10e6 cells)
Application Notes:
The stated application concentrations are suggested starting points. Titration of the VIL1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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