A portion of amino acids 451-479 from the human protein were used as the immunogen for the ATP1A2 antibody.
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium, providing the energy for active transport of various nutrients. [UniProt]
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25,Immunofluorescence: 1:25,Flow cytometry: 1:25 (1x10e6 cells)
Application Notes:
The stated application concentrations are suggested starting points. Titration of the ATP1A2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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