A portion of amino acids 2-36 from the N-terminal region of the human protein was used as the immunogen for the METTL14 antibody.
N6-adenosine-methyltransferase subunit METTL14 is an N6-methyltransferase that methylates adenosine residues of some mRNAs and acts as a regulator of the circadian clock and self-renewal of embryonic stem cells. N6-methyladenosine (m6A), which takes place at the 5-[AG]GAC-3 consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing and processing and mRNA stability. M6A regulates the length of the circadian clock: acts as a early pace-setter in the circadian loop M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding developmental regulators, results in transcript destabilization, maintaining the groung state of ESCs, thereby promoting self-renewal of ESCs.
The stated application concentrations are suggested starting points. Titration of the METTL14 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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