A portion of amino acids 170-204 from human Factor XIIIa was used as the immunogen for the Factor XIIIa antibody.
Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl- epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.
Western blot: 1:1000-1:2000,Flow cytometry: 1:25,Immunofluorescence: 1:25
Application Notes:
The stated application concentrations are suggested starting points. Titration of the Factor XIIIa antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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