A portion of amino acids 643-677 from human COL14A1 was used as the immunogen for the COL14A1 antibody.
Plays an adhesive role by integrating collagen bundles. It is probably associated with the surface of interstitial collagen fibrils via COL1. The COL2 domain may then serve as a rigid arm which sticks out from the fibril and protrudes the large N-terminal globular domain into the extracellular space, where it might interact with other matrix molecules or cell surface receptors (By similarity).
The stated application concentrations are suggested starting points. Titration of the COL14A1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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