A synthetic PKC alpha/aPKC peptide was used as the immunogen for the PKC alpha antibody.
This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
The stated application concentrations are suggested starting points. Titration of the PKC alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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