A human recombinant partial protein was used as the immunogen for this Vimentin antibody.
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Western blot: 1:1000,ICC/IF: 1:800,Immunoprecipitation: suitable
Application Notes:
The stated application concentrations are suggested starting points. Titration of the Vimentin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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