Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0)]. Not yet tested in other applications. Optima
Sample (30 ug of whole cell lysate) A: IMR32 7.5% SDS PAGE ZP2 antibody diluted at 1:1000
IHC of paraffin-embedded SCC4 xenograft, using ZP2 antibody at 1:250 dilution.
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