Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Sample (30 ug of whole cell lysate) A: 293T B: Raji 15% SDS PAGE YAF2 antibody diluted at 1:500
Immunofluorescence of paraformaldehyde-fixed HeLa, using YAF2 antibody at 1:500 dilution.
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