0.1 M Tris, pH 7, 0.1 M Glycine, 20% Glycerol, 0.01% Thimerosal
Purity:
Immunoaffinity purified
Form:
0.1 M Tris, pH 7, 0.1 M Glycine, 20% Glycerol, 0.01% Thimerosal
Application Dilute:
IHC, IHC-P (1:100 - 1:1000), WB (1:500 - 1:3000)
Application Notes:
Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000[suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0)]. Not yet tested in other applications. Optimal
Sample (30 ug of whole cell lysate) A: THP-1 12% SDS PAGE SH3BP5 antibody diluted at 1:1000
IHC of paraffin-embedded Colon carcinoma, using SH3BP5 antibody at 1:250 dilution.
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