0.1 M Tris-glycine, pH 7.0, 20% glycerol, 0.01% Thimerosal
Purity:
Immunoaffinity purified
Form:
0.1 M Tris-glycine, pH 7.0, 20% glycerol, 0.01% Thimerosal
Application Dilute:
IHC, IHC-P (1:100 - 1:1000), WB (1:500 - 1:3000)
Application Notes:
Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Sample (30 ug of whole cell lysate) A: Jurkat 10% SDS PAGE HAUS4 antibody diluted at 1:1000
IHC of paraffin-embedded Colon ca, using C14orf94 antibody at 1:500 dilution.
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