Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0)]. Not yet tested in other applications. Optima
Sample (30 ug of whole cell lysate) A: HL-60 7.5% SDS PAGE CD180 / RP105 antibody diluted at 1:1000
IHC of paraffin-embedded CL1-5 xenograft, using RP105 antibody at 1:500 dilution.
* VAT and and shipping costs not included. Errors and price changes excepted