T4 RNA Ligase II, also known as T4 Rnl2 (gp24.1), is an ATP-dependent RNA ligase catalyzing the inter- and intramolecular RNA strand joining activity via phosphodiester bond formation. Unlike T4 RNA Ligase I, T4 RNA Ligase II is much more active in joining nicks on double-stranded RNA (dsRNA) than joining the ends of single-stranded RNA. The enzyme can also ligate the 3´ OH of RNA to the 5´ phosphate of DNA in a double-stranded structure. The enzyme requires an adjacent 5´ phosphate and 3´ OH for ligation.
Buffer:
Liquid
T4 RNA Ligase II on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Ligation of a 22mer RNA substrate using T4 RNA ligase II, followed by Urea page gel analysis.
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