Non ionic detergents like Brig 35 are used in immunoassays for diluting antibodies to lower background, and non-specific ag-ab reaction. This detergent is also used in washing buffer from 0.5- 0.1% in all immunoassays (ELISA, Immunohistochemistry (IHC), Blot assays and others) to reduce background. Brig 35 is polyoxyethylene laural ether, it lower surface tension of water.
Buffer:
This detergent is supplied as 15% in water with 0.15 % Proclin 300 as preservative.
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