Urease (EC 3.5.1.5) is an enzyme molecular weight 480 kDa that catalyzes the hydrolysis of urea into carbon dioxide and ammonia. The reaction occurs as follows: (NH2)2CO + H2O CO2 + 2NH3 The biotin label is separated from the protein core by a caproyl spacer.
Concentration:
2.5 mg/ml
Buffer:
50 mM PO4, 150 mM NaCl, 40 % glycerol, 1 mM EDTA 0.05% azide, pH 7.4
Source:
Urease is purified from jack beans
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