Streptavidin is a 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC).
Buffer:
The complex is supplied in one ml of 10 mM Tris-HCl, 200 mM NaCl, 50% glycerol, pH8.0 buffer containing 10 mg of BSA and 0.05% sodium azide
