SDF1 (12B8) Rabbit Monoclonal Antibody, Unconjugated
Catalog Number:
EKL-AMRE17675
Product Overview
Article Name:
SDF1 (12B8) Rabbit Monoclonal Antibody, Unconjugated
Biozol Catalog Number:
EKL-AMRE17675
Supplier Catalog Number:
AMRe17675
Alternative Catalog Number:
EKL-AMRE17675-50UL, EKL-AMRE17675-100UL, EKL-AMRE17675-200UL
Manufacturer:
EnkiLife
Host:
Rabbit
Category:
Antikörper
Application:
FC, ICC, WB
Species Reactivity:
Human, Mouse
Conjugation:
Unconjugated
Alternative Names:
Stromal cell-derived factor 1, SDF-1, hSDF-1, IRH, hIRH, PBSF, CXCL12, SDF1, SDF1A, SDF1B,
Product Description
Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. Chemoattractant active on T-lymphocytes and monocytes but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. Binds to the allosteric site (site 2) of integrins and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB1 in a CXCR4-independent manner (PubMed:29301984). Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T- lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development, required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. Stimulates the proliferation of bone marrow-derived B-cell progenitors in the presence of IL7 as well as growth of stromal cell- dependent pre-B-cells (By similarity).
Product Properties
Clonality:
Monoclonal
Molecular Weight:
11kDa
NCBI:
6387
UniProt:
P48061
Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4C short term. Store at -20C long term. Avoid freeze / thaw cycle.
Purity:
Affinity purification
Form:
Liquid
Target:
CXCL12
Application Notes
Application Dilute:
WB 1:2000-1:20000,ICC/IF 1:100-1:200,FC 1:20-1:50
