TNFRSF10A Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
CSB-PA023964LA01HU
- Images (5)
| Article Name: | TNFRSF10A Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | CSB-PA023964LA01HU |
| Supplier Catalog Number: | CSB-PA023964LA01HU |
| Alternative Catalog Number: | CSB-PA023964LA01HU-100UG, CSB-PA023964LA01HU-50UG |
| Manufacturer: | Cusabio |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC, IP, WB |
| Species Reactivity: | Human |
| Conjugation: | Unconjugated |
| Alternative Names: | Apo2 antibody, CD261 antibody, Cytotoxic TRAIL receptor antibody, Death receptor 4 antibody, DR4 antibody, NF related apoptosis-inducing ligand receptor 1 antibody, TNF receptor superfamily member 10a antibody, TNF-related apoptosis-inducing ligand receptor 1 antibody, TNFRSF10A antibody, TR10A_HUMAN antibody, TRAIL receptor 1 antibody, TRAIL-R1 antibody, TRAILR 1 antibody, TRAILR1 antibody, Tumor necrosis factor receptor superfamily member 10A antibody, Tumor necrosis factor receptor superfamily member 10a variant 2 antibody, Tumor necrosis factor receptor superfamily, member 10a antibody |
| Clonality: | Polyclonal |
| UniProt: | O00220 |
| Buffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purity: | >95%, Protein G purified |
| Form: | Liquid |
| Target: | TNFRSF10A |
| Application Dilute: | Recommended dilution: WB:1:1000-1:5000, IHC:1:100-1:500, IF:1:50-1:500, IP:1:200-1:2000 |
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Immunofluorescent analysis of A549 cells using CSB-PA023964LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) |
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IHC image of CSB-PA023964LA01HU diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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IHC image of CSB-PA023964LA01HU diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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Immunoprecipitating TNFRSF10A in 293 whole cell lysate Lane 1: Rabbit control IgG (1ug) instead of CSB-PA023964LA01HU in 293 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA023964LA01HU (6ug) + 293 whole cell lysate (500ug) Lane 3: 293 whole cell lysate (10ug) |
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Western Blot Positive WB detected in: HEK293 whole cell lysate All lanes: TNFRSF10A antibody at 3.2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 51 kDa Observed band size: 51 kDa |
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