TIMM23 Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
CSB-PA023553ESR2HU
- Images (5)
| Article Name: | TIMM23 Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | CSB-PA023553ESR2HU |
| Supplier Catalog Number: | CSB-PA023553ESR2HU |
| Alternative Catalog Number: | CSB-PA023553ESR2HU-100UL, CSB-PA023553ESR2HU-50UL |
| Manufacturer: | Cusabio |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, WB |
| Species Reactivity: | Human, Mouse |
| Conjugation: | Unconjugated |
| Alternative Names: | TIMM23 antibody, TIM23 antibody, Mitochondrial import inner membrane translocase subunit Tim23 antibody |
| Clonality: | Polyclonal |
| UniProt: | O14925 |
| Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purity: | Antigen Affinity Purified |
| Form: | Liquid |
| Target: | TIMM23 |
| Application Dilute: | Recommended dilution: WB:1:500-1:5000,IF:1:20-1:200 |
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Immunofluorescence staining of A549 cell with CSB-PA023553ESR2HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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Western Blot Positive WB detected in: JK whole cell lysate(20µg), MCF-7 whole cell lysate(20µg), 293 whole cell lysate(20µg), A549 whole cell lysate(20µg), Hela whole cell lysate(20µg), Mouse Heart tissue lysate(20µg) All lanes: TIMM23 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 300s |
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