RORC Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA020071LA01HU
Article Name: RORC Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA020071LA01HU
Supplier Catalog Number: CSB-PA020071LA01HU
Alternative Catalog Number: CSB-PA020071LA01HU-100UG, CSB-PA020071LA01HU-50UG
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IHC, WB
Species Reactivity: Human
Conjugation: Unconjugated
Alternative Names: IMD42 antibody, MGC129539 antibody, NR1F3 antibody, Nuclear receptor ROR gamma antibody, Nuclear receptor ROR-gamma antibody, Nuclear receptor RZR gamma antibody, Nuclear receptor RZR-gamma antibody, Nuclear receptor subfamily 1 group F member 3 antibody, RAR related orphan nuclear receptor variant 2 antibody, RAR related orphan receptor C antibody, RAR related orphan receptor C, isoform a antibody, RAR related orphan receptor gamma antibody, RAR-related orphan receptor C antibody, Retinoic acid binding receptor gamma antibody, Retinoid related orphan receptor gamma antibody, Retinoid-related orphan receptor-gamma antibody, Rorc antibody, RORG antibody, RORG_HUMAN antibody, RZR GAMMA antibody, RZRG antibody, TOR antibody
Clonality: Polyclonal
UniProt: P51449
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purity: >95%, Protein G purified
Form: Liquid
Target: RORC
Application Dilute: Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200
IHC image of CSB-PA020071LA01HU diluted at 1:1000 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody
IHC image of CSB-PA020071LA01HU diluted at 1:1000 and staining in paraffin-embedded human skin tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-PA020071LA01HU diluted at 1:1000 and staining in paraffin-embedded mouse liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Western Blot
Positive WB detected in: HepG2 whole cell lysate
All lanes: RORC antibody at 5.2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 59, 56 kDa
Observed band size: 59 kDa
Western Blot
Positive WB detected in: HT29 whole cell lysate, MCF7 whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate
All lanes: RORC antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 59, 56 kDa
Observed band size: 59 kDa